3RTO

Acoustically mounted porcine insulin microcrystals yield an X-ray SAD structure

3RTO の概要

• エントリーDOI: 10.2210/pdb3rto/pdb
• 分子名称: Insulin, ZINC ION, ... (4 entities in total)
• 機能のキーワード: hormone
• 由来する生物種: Sus scrofa (pig); 詳細
• 細胞内の位置: Secreted: P01315 P01315
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 11706.07

構造登録者

Soares, A.S.,Engel, M.A.,Stearns, R.,Datwani, S.,Olechno, J.,Ellson, R.,Skinner, J.M.,Allaire, M.,Orville, A.M. (登録日: 2011-05-03, 公開日: 2011-05-18, 最終更新日: 2024-11-06)

主引用文献

Soares, A.S.,Engel, M.A.,Stearns, R.,Datwani, S.,Olechno, J.,Ellson, R.,Skinner, J.M.,Allaire, M.,Orville, A.M.
Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures.
Biochemistry, 50:4399-4401, 2011

PubMed Abstract: We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.

PubMed: 21542590
DOI: 10.1021/bi200549x

実験手法

X-RAY DIFFRACTION (1.8 Å)