3RPT

Crystal structure of the anti-HIV b12 scaffold protein

3RPT の概要

• エントリーDOI: 10.2210/pdb3rpt/pdb
• 分子名称: Endoglucanase E-2, SULFATE ION (3 entities in total)
• 機能のキーワード: scaffold protein anti-hiv, hydrolase
• 由来する生物種: Thermobifida fusca; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59747.73

構造登録者

Chen, L.,Kwong, P.D. (登録日: 2011-04-27, 公開日: 2011-12-21, 最終更新日: 2017-07-26)

主引用文献

Azoitei, M.L.,Correia, B.E.,Ban, Y.E.,Carrico, C.,Kalyuzhniy, O.,Chen, L.,Schroeter, A.,Huang, P.S.,McLellan, J.S.,Kwong, P.D.,Baker, D.,Strong, R.K.,Schief, W.R.
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
Science, 334:373-376, 2011

PubMed Abstract: The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.

PubMed: 22021856
DOI: 10.1126/science.1209368

実験手法

X-RAY DIFFRACTION (1.303 Å)