3RPB

THE C2B-DOMAIN OF RABPHILIN: STRUCTURAL VARIATIONS IN A JANUS-FACED DOMAIN

3RPB の概要

• エントリーDOI: 10.2210/pdb3rpb/pdb
• 分子名称: RABPHILIN 3-A (1 entity in total)
• 機能のキーワード: c2-domains, c2b-domain, rabphilin, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cell junction, synapse (By similarity): P47709
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16253.78

構造登録者

Ubach, J.,Garcia, J.,Nittler, M.P.,Sudhof, T.C.,Rizo, J. (登録日: 1999-04-19, 公開日: 1999-12-23, 最終更新日: 2024-05-22)

主引用文献

Ubach, J.,Garcia, J.,Nittler, M.P.,Sudhof, T.C.,Rizo, J.
Structure of the Janus-faced C2B domain of rabphilin.
Nat.Cell Biol., 1:106-112, 1999

PubMed Abstract: C2 domains are widespread protein modules that often occur as tandem repeats in many membrane-trafficking proteins such as synaptotagmin and rabphilin. The first and second C2 domains (C2A and C2B, respectively) have a high degree of homology but also specific differences. The structure of the C2A domain of synaptotagmin I has been extensively studied but little is known about the C2B domains. We have used NMR spectroscopy to determine the solution structure of the C2B domain of rabphilin. The overall structure of the C2B domain is very similar to that of other C2 domains, with a rigid beta-sandwich core and loops at the top (where Ca2+ binds) and the bottom. Surprisingly, a relatively long alpha-helix is inserted at the bottom of the domain and is conserved in all C2B domains. Our results, together with the Ca(2+)-independent interactions observed for C2B domains, indicate that these domains have a Janus-faced nature, with a Ca(2+)-binding top surface and a Ca(2+)-independent bottom surface.

PubMed: 10559882
DOI: 10.1038/10076

実験手法

SOLUTION NMR