3ROZ
Crystal Structure of Mouse Apolipoprotein A-I Binding Protein in Complex with Nicotinamide
Summary for 3ROZ
| Entry DOI | 10.2210/pdb3roz/pdb |
| Related | 2DG2 2O8N 3RNO 3RO7 3ROE 3ROG 3ROX |
| Descriptor | Apolipoprotein A-I-binding protein, SULFATE ION, NICOTINAMIDE (3 entities in total) |
| Functional Keywords | rossmann fold, protein binding |
| Biological source | Mus musculus (mouse) |
| Cellular location | Mitochondrion (By similarity): Q8K4Z3 |
| Total number of polymer chains | 1 |
| Total formula weight | 30091.15 |
| Authors | Shumilin, I.A.,Jha, K.N.,Cymborowski, M.,Herr, J.C.,Minor, W. (deposition date: 2011-04-26, release date: 2012-07-18, Last modification date: 2024-11-20) |
| Primary citation | Shumilin, I.A.,Cymborowski, M.,Chertihin, O.,Jha, K.N.,Herr, J.C.,Lesley, S.A.,Joachimiak, A.,Minor, W. Identification of unknown protein function using metabolite cocktail screening. Structure, 20:1715-1725, 2012 Cited by PubMed Abstract: Proteins of unknown function comprise a significant fraction of sequenced genomes. Defining the roles of these proteins is vital to understanding cellular processes. Here, we describe a method to determine a protein function based on the identification of its natural ligand(s) by the crystallographic screening of the binding of a metabolite library, followed by a focused search in the metabolic space. The method was applied to two protein families with unknown function, PF01256 and YjeF_N. The PF01256 proteins, represented by YxkO from Bacillus subtilis and the C-terminal domain of Tm0922 from Thermotoga maritima, were shown to catalyze ADP/ATP-dependent NAD(P)H-hydrate dehydratation, a previously described orphan activity. The YjeF_N proteins, represented by mouse apolipoprotein A-I binding protein and the N-terminal domain of Tm0922, were found to interact with an adenosine diphosphoribose-related substrate and likely serve as ADP-ribosyltransferases. Crystallographic screening of metabolites serves as an efficient tool in functional analyses of uncharacterized proteins. PubMed: 22940582DOI: 10.1016/j.str.2012.07.016 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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