3ROZ

Crystal Structure of Mouse Apolipoprotein A-I Binding Protein in Complex with Nicotinamide

3ROZ の概要

• エントリーDOI: 10.2210/pdb3roz/pdb
• 関連するPDBエントリー: 2DG2 2O8N 3RNO 3RO7 3ROE 3ROG 3ROX
• 分子名称: Apolipoprotein A-I-binding protein, SULFATE ION, NICOTINAMIDE (3 entities in total)
• 機能のキーワード: rossmann fold, protein binding
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Mitochondrion (By similarity): Q8K4Z3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30091.15

構造登録者

Shumilin, I.A.,Jha, K.N.,Cymborowski, M.,Herr, J.C.,Minor, W. (登録日: 2011-04-26, 公開日: 2012-07-18, 最終更新日: 2024-11-20)

主引用文献

Shumilin, I.A.,Cymborowski, M.,Chertihin, O.,Jha, K.N.,Herr, J.C.,Lesley, S.A.,Joachimiak, A.,Minor, W.
Identification of unknown protein function using metabolite cocktail screening.
Structure, 20:1715-1725, 2012

PubMed Abstract: Proteins of unknown function comprise a significant fraction of sequenced genomes. Defining the roles of these proteins is vital to understanding cellular processes. Here, we describe a method to determine a protein function based on the identification of its natural ligand(s) by the crystallographic screening of the binding of a metabolite library, followed by a focused search in the metabolic space. The method was applied to two protein families with unknown function, PF01256 and YjeF_N. The PF01256 proteins, represented by YxkO from Bacillus subtilis and the C-terminal domain of Tm0922 from Thermotoga maritima, were shown to catalyze ADP/ATP-dependent NAD(P)H-hydrate dehydratation, a previously described orphan activity. The YjeF_N proteins, represented by mouse apolipoprotein A-I binding protein and the N-terminal domain of Tm0922, were found to interact with an adenosine diphosphoribose-related substrate and likely serve as ADP-ribosyltransferases. Crystallographic screening of metabolites serves as an efficient tool in functional analyses of uncharacterized proteins.

PubMed: 22940582
DOI: 10.1016/j.str.2012.07.016

実験手法

X-RAY DIFFRACTION (2.8 Å)