3ROH

Crystal Structure of Leukotoxin (LukE) from Staphylococcus aureus subsp. aureus COL.

3ROH の概要

• エントリーDOI: 10.2210/pdb3roh/pdb
• 分子名称: Leucotoxin LukEv, TRIETHYLENE GLYCOL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: structural genomics, center for structural genomics of infectious diseases, csgid, leukocidin-like, leukocidin, pore-forming toxin, membrane and cell surface proteins and peptides, toxin
• 由来する生物種: Staphylococcus aureus subsp. aureus NCTC 8325
• 細胞内の位置: Secreted : Q2FXB0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37259.57

構造登録者

Minasov, G.,Halavaty, A.,Shuvalova, L.,Dubrovska, I.,Winsor, J.,Bagnoli, F.,Falugi, F.,Bottomley, M.,Grandi, G.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2011-04-25, 公開日: 2011-05-04, 最終更新日: 2023-09-13)

主引用文献

Nocadello, S.,Minasov, G.,Shuvalova, L.,Dubrovska, I.,Sabini, E.,Bagnoli, F.,Grandi, G.,Anderson, W.F.
Crystal structures of the components of the Staphylococcus aureus leukotoxin ED.
Acta Crystallogr.,Sect.D, 72:113-120, 2016

PubMed Abstract: Staphylococcal leukotoxins are a family of β-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins that Staphylococcus aureus produces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays in S. aureus pathogenesis and the identification of its protein receptors, combined with its presence in S. aureus methicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. The two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.

PubMed: 26894539
DOI: 10.1107/S2059798315023207

実験手法

X-RAY DIFFRACTION (3.2 Å)