3RKP

Crystal structure of BcpA*(D312A), the major pilin subunit of Bacillus cereus

3RKP の概要

• エントリーDOI: 10.2210/pdb3rkp/pdb
• 関連するPDBエントリー: 3KPT
• 分子名称: Collagen adhesion protein (2 entities in total)
• 機能のキーワード: intramolecular amide bond, jelly-roll, ig, pilin subunit, cell adhesion
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78467.16

構造登録者

Hendrickx, A.P.,Poor, C.B.,Jureller, J.E.,Budzik, J.M.,He, C.,Schneewind, O. (登録日: 2011-04-18, 公開日: 2012-05-30, 最終更新日: 2024-11-20)

主引用文献

Hendrickx, A.P.,Poor, C.B.,Jureller, J.E.,Budzik, J.M.,He, C.,Schneewind, O.
Isopeptide bonds of the major pilin protein BcpA influence pilus structure and bundle formation on the surface of Bacillus cereus.
Mol.Microbiol., 85:152-163, 2012

PubMed Abstract: Bacillus cereus strains elaborate pili on their surface using a mechanism of sortase-mediated cross-linking of major and minor pilus components. Here we used a combination of electron microscopy and atomic force microscopy to visualize these structures. Pili occur as single, double or higher order assemblies of filaments formed from monomers of the major pilin, BcpA, capped by the minor pilin, BcpB. Previous studies demonstrated that within assembled pili, four domains of BcpA - CNA(1), CNA(2), XNA and CNA(3) - each acquire intramolecular lysine-asparagine isopeptide bonds formed via catalytic glutamic acid or aspartic acid residues. Here we showed that mutants unable to form the intramolecular isopeptide bonds in the CNA(2) or CNA(3) domains retain the ability to form pilus bundles. A mutant lacking the CNA(1) isopeptide bond assembled deformed pilin subunits that failed to associate as bundles. X-ray crystallography revealed that the BcpA variant Asp(312) Ala, lacking an aspartyl catalyst, did not generate the isopeptide bond within the jelly-roll structure of XNA. The Asp(312) Ala mutant was also unable to form bundles and promoted the assembly of deformed pili. Thus, structural integrity of the CNA(1) and XNA domains are determinants for the association of pili into higher order bundle structures and determine native pilus structure.

PubMed: 22624947
DOI: 10.1111/j.1365-2958.2012.08098.x

実験手法

X-RAY DIFFRACTION (2.243 Å)