3RIO

Crystal structure of GlcT CAT-PRDI

Summary for 3RIO

Entry DOI	10.2210/pdb3rio/pdb
Descriptor	PtsGHI operon antiterminator, GLYCEROL (3 entities in total)
Functional Keywords	twisted beta sheet, four helix bundle, transcriptional antitermination, transcription
Biological source	Bacillus subtilis
Total number of polymer chains	1
Total formula weight	21191.25
Authors	Himmel, S.,Grosse, C.,Wolff, S.,Becker, S. (deposition date: 2011-04-14, release date: 2012-05-09, Last modification date: 2024-02-28)
Primary citation	Himmel, S.,Grosse, C.,Wolff, S.,Schwiegk, C.,Becker, S. Structure of the RBD-PRDI fragment of the antiterminator protein GlcT. Acta Crystallogr.,Sect.F, 68:751-756, 2012 Cited by PubMed Abstract: GlcT is a transcriptional antiterminator protein that is involved in regulation of glucose metabolism in Bacillus subtilis. Antiterminator proteins bind specific RNA sequences, thus preventing the formation of overlapping terminator stem-loops. The structure of a fragment (residues 3-170) comprising the RNA-binding domain (RBD) and the first regulatory domain (PRDI) of GlcT was solved at 2.0 Å resolution with one molecule in the asymmetric unit. The two domains are connected by a helical linker. Their interface is mostly constituted by hydrophobic interactions. PubMed: 22750856 DOI: 10.1107/S1744309112020635 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.99 Å)

Structure validation