3RB8

Structure of the phage tubulin PhuZ(SeMet)-GDP

3RB8 の概要

• エントリーDOI: 10.2210/pdb3rb8/pdb
• 関連するPDBエントリー: 3R4V
• 分子名称: Putative uncharacterized protein, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: tubulin, unknown function
• 由来する生物種: Pseudomonas phage 201phi2-1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35458.12

構造登録者

Agard, D.A.,Pogliano, J.,Kraemer, J.A.,Erb, M.L.,Waddling, C.A.,Montabana, E.A.,Wang, H.,Nguyen, K.,Pham, S. (登録日: 2011-03-28, 公開日: 2012-07-04, 最終更新日: 2024-11-20)

主引用文献

Kraemer, J.A.,Erb, M.L.,Waddling, C.A.,Montabana, E.A.,Zehr, E.A.,Wang, H.,Nguyen, K.,Pham, D.S.,Agard, D.A.,Pogliano, J.
A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell.
Cell(Cambridge,Mass.), 149:1488-1499, 2012

PubMed Abstract: Tubulins are essential for the reproduction of many eukaryotic viruses, but historically, bacteriophage were assumed not to require a cytoskeleton. Here, we identify a tubulin-like protein, PhuZ, from bacteriophage 201φ2-1 and show that it forms filaments in vivo and in vitro. The PhuZ structure has a conserved tubulin fold, with an unusual, extended C terminus that we demonstrate to be critical for polymerization in vitro and in vivo. Longitudinal packing in the crystal lattice mimics packing observed by EM of in-vitro-formed filaments, indicating how interactions between the C terminus and the following monomer drive polymerization. PhuZ forms a filamentous array that is required for positioning phage DNA within the bacterial cell. Correct positioning to the cell center and optimal phage reproduction only occur when the PhuZ filament is dynamic. Thus, we show that PhuZ assembles a spindle-like array that functions analogously to the microtubule-based spindles of eukaryotes.

PubMed: 22726436
DOI: 10.1016/j.cell.2012.04.034

実験手法

X-RAY DIFFRACTION (2.6 Å)