3R8F

Replication initiator DnaA bound to AMPPCP and single-stranded DNA

3R8F の概要

• エントリーDOI: 10.2210/pdb3r8f/pdb
• 分子名称: Chromosomal replication initiator protein dnaA, 5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3', MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: aaa+ atpase, replication initiator, dna binding, replication activator-dna complex, replication activator/dna
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cytoplasm: O66659
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 157279.51

構造登録者

Duderstadt, K.E.,Chuang, K.,Berger, J.M. (登録日: 2011-03-23, 公開日: 2011-09-28, 最終更新日: 2023-09-13)

主引用文献

Duderstadt, K.E.,Chuang, K.,Berger, J.M.
DNA stretching by bacterial initiators promotes replication origin opening.
Nature, 478:209-213, 2011

PubMed Abstract: Many replication initiators form higher-order oligomers that process host replication origins to promote replisome formation. In addition to dedicated duplex-DNA-binding domains, cellular initiators possess AAA+ (ATPases associated with various cellular activities) elements that drive functions ranging from protein assembly to origin recognition. In bacteria, the AAA+ domain of the initiator DnaA has been proposed to assist in single-stranded DNA formation during origin melting. Here we show crystallographically and in solution that the ATP-dependent assembly of Aquifex aeolicus DnaA into a spiral oligomer creates a continuous surface that allows successive AAA+ domains to bind and extend single-stranded DNA segments. The mechanism of binding is unexpectedly similar to that of RecA, a homologous recombination factor, but it differs in that DnaA promotes a nucleic acid conformation that prevents pairing of a complementary strand. These findings, combined with strand-displacement assays, indicate that DnaA opens replication origins by a direct ATP-dependent stretching mechanism. Comparative studies reveal notable commonalities between the approach used by DnaA to engage DNA substrates and other, nucleic-acid-dependent, AAA+ systems.

PubMed: 21964332
DOI: 10.1038/nature10455

実験手法

X-RAY DIFFRACTION (3.366 Å)