3R50
Structure analysis of a wound-inducible lectin ipomoelin from sweet potato
3R50 の概要
エントリーDOI | 10.2210/pdb3r50/pdb |
関連するPDBエントリー | 3R51 3R52 3R53 |
分子名称 | Ipomoelin (2 entities in total) |
機能のキーワード | beta prism, carbohydrate/sugar binding, carbohydrate-binding protein |
由来する生物種 | Ipomoea batatas (batate) |
タンパク質・核酸の鎖数 | 5 |
化学式量合計 | 86727.10 |
構造登録者 | |
主引用文献 | Chang, W.C.,Liu, K.L.,Hsu, F.C.,Jeng, S.T.,Cheng, Y.S. Ipomoelin, a jacalin-related lectin with a compact tetrameric association and versatile carbohydrate binding properties regulated by its N terminus. Plos One, 7:e40618-e40618, 2012 Cited by PubMed Abstract: Many proteins are induced in the plant defense response to biotic stress or mechanical wounding. One group is lectins. Ipomoelin (IPO) is one of the wound-inducible proteins of sweet potato (Ipomoea batatas cv. Tainung 57) and is a Jacalin-related lectin (JRL). In this study, we resolved the crystal structures of IPO in its apo form and in complex with carbohydrates such as methyl α-D-mannopyranoside (Me-Man), methyl α-D-glucopyranoside (Me-Glc), and methyl α-D-galactopyranoside (Me-Gal) in different space groups. The packing diagrams indicated that IPO might represent a compact tetrameric association in the JRL family. The protomer of IPO showed a canonical β-prism fold with 12 strands of β-sheets but with 2 additional short β-strands at the N terminus. A truncated IPO (ΔN10IPO) by removing the 2 short β-strands of the N terminus was used to reveal its role in a tetrameric association. Gel filtration chromatography confirmed IPO as a tetrameric form in solution. Isothermal titration calorimetry determined the binding constants (K(A)) of IPO and ΔN10IPO against various carbohydrates. IPO could bind to Me-Man, Me-Glc, and Me-Gal with similar binding constants. In contrast, ΔN10IPO showed high binding ability to Me-Man and Me-Glc but could not bind to Me-Gal. Our structural and functional analysis of IPO revealed that its compact tetrameric association and carbohydrate binding polyspecificity could be regulated by the 2 additional N-terminal β-strands. The versatile carbohydrate binding properties of IPO might play a role in plant defense. PubMed: 22808208DOI: 10.1371/journal.pone.0040618 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.27 Å) |
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