3R4F

Prohead RNA

3R4F の概要

• エントリーDOI: 10.2210/pdb3r4f/pdb
• 分子名称: pRNA, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: prohead rna, motor, viral packaging, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21408.23

構造登録者

Ding, F.,Lu, C.,Zhano, W.,Rajashankar, K.R.,Anderson, D.L.,Jardine, P.J.,Grimes, S.,Ke, A. (登録日: 2011-03-17, 公開日: 2011-04-20, 最終更新日: 2024-02-21)

主引用文献

Ding, F.,Lu, C.,Zhao, W.,Rajashankar, K.R.,Anderson, D.L.,Jardine, P.J.,Grimes, S.,Ke, A.
Structure and assembly of the essential RNA ring component of a viral DNA packaging motor.
Proc.Natl.Acad.Sci.USA, 108:7357-7362, 2011

PubMed Abstract: Prohead RNA (pRNA) is an essential component in the assembly and operation of the powerful bacteriophage 29 DNA packaging motor. The pRNA forms a multimeric ring via intermolecular base-pairing interactions between protomers that serves to guide the assembly of the ring ATPase that drives DNA packaging. Here we report the quaternary structure of this rare multimeric RNA at 3.5 Å resolution, crystallized as tetrameric rings. Strong quaternary interactions and the inherent flexibility helped rationalize how free pRNA is able to adopt multiple oligomerization states in solution. These characteristics also allowed excellent fitting of the crystallographic pRNA protomers into previous prohead/pRNA cryo-EM reconstructions, supporting the presence of a pentameric, but not hexameric, pRNA ring in the context of the DNA packaging motor. The pentameric pRNA ring anchors itself directly to the phage prohead by interacting specifically with the fivefold symmetric capsid structures that surround the head-tail connector portal. From these contacts, five RNA superhelices project from the pRNA ring, where they serve as scaffolds for binding and assembly of the ring ATPase, and possibly mediate communication between motor components. Construction of structure-based designer pRNAs with little sequence similarity to the wild-type pRNA were shown to fully support the packaging of 29 DNA.

PubMed: 21471452
DOI: 10.1073/pnas.1016690108

実験手法

X-RAY DIFFRACTION (3.5 Å)