3QY3

PA2801 protein, a putative Thioesterase from Pseudomonas aeruginosa

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3QY3 の概要

• エントリーDOI: 10.2210/pdb3qy3/pdb
• 分子名称: Thioesterase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: structural genomics, psi, protein structure initiative, midwest center for structural genomics, mcsg, hydrolase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17887.94

構造登録者

Osipiuk, J.,Xu, X.,Savchenko, A.,Edwards, A.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (登録日: 2011-03-02, 公開日: 2011-03-16, 最終更新日: 2024-10-16)

主引用文献

Gonzalez, C.F.,Tchigvintsev, A.,Brown, G.,Flick, R.,Evdokimova, E.,Xu, X.,Osipiuk, J.,Cuff, M.E.,Lynch, S.,Joachimiak, A.,Savchenko, A.,Yakunin, A.F.
Structure and activity of the Pseudomonas aeruginosa hotdog-fold thioesterases PA5202 and PA2801.
Biochem.J., 444:445-455, 2012

PubMed Abstract: The hotdog fold is one of the basic protein folds widely present in bacteria, archaea and eukaryotes. Many of these proteins exhibit thioesterase activity against fatty acyl-CoAs and play important roles in lipid metabolism, cellular signalling and degradation of xenobiotics. The genome of the opportunistic pathogen Pseudomonas aeruginosa contains over 20 genes encoding predicted hotdog-fold proteins, none of which have been experimentally characterized. We have found that two P. aeruginosa hotdog proteins display high thioesterase activity against 3-hydroxy-3-methylglutaryl-CoA and glutaryl-CoA (PA5202), and octanoyl-CoA (PA2801). Crystal structures of these proteins were solved (at 1.70 and 1.75 Å for PA5202 and PA2801 respectively) and revealed a hotdog fold with a potential catalytic carboxylate residue located on the long α-helix (Asp(57) in PA5202 and Glu(35) in PA2801). Alanine residue replacement mutagenesis of PA5202 identified four residues (Asn(42), Arg(43), Asp(57) and Thr(76)) that are critical for its activity and are located in the active site. A P. aeruginosa PA5202 deletion strain showed an increased secretion of the antimicrobial pigment pyocyanine and an increased expression of genes involved in pyocyanin biosynthesis, suggesting a functional link between PA5202 activity and pyocyanin production. Thus the P. aeruginosa hotdog thioesterases PA5202 and PA2801 have similar structures, but exhibit different substrate preferences and functions.

PubMed: 22439787
DOI: 10.1042/BJ20112032

実験手法

X-RAY DIFFRACTION (1.75 Å)