3QTB

Structure of the universal stress protein from Archaeoglobus fulgidus in complex with dAMP

Summary for 3QTB

• Entry DOI: 10.2210/pdb3qtb/pdb
• Related: 3DLO
• Descriptor: Uncharacterized protein, 2'-DEOXYADENOSINE-5'-MONOPHOSPHATE, ACETATE ION, ... (4 entities in total)
• Functional Keywords: structural genomics, psi-biology, midwest center for structural genomics, mcsg, a/b class, rfm like, putative universal stress protein, unknown function
• Biological source: Archaeoglobus fulgidus
• Total number of polymer chains: 2
• Total formula weight: 35187.34

Authors

Tkaczuk, K.L.,Shumilin, I.A.,Chruszcz, M.,Cymborowski, M.,Xu, X.,Di Leo, R.,Savchenko, A.,Joachimiak, A.,Minor, W.,Midwest Center for Structural Genomics (MCSG) (deposition date: 2011-02-22, release date: 2011-03-30, Last modification date: 2024-10-16)

Primary citation

Tkaczuk, K.L.,Shumilin, I.A.,Chruszcz, M.,Evdokimova, E.,Savchenko, A.,Minor, W.
Structural and functional insight into the universal stress protein family.
Evol Appl, 6:434-449, 2013

PubMed Abstract: We present the crystal structures of two universal stress proteins (USP) from Archaeoglobus fulgidus and Nitrosomonas europaea in both apo- and ligand-bound forms. This work is the first complete synthesis of the structural properties of 26 USP available in the Protein Data Bank, over 75% of which were determined by structure genomics centers with no additional information provided. The results of bioinformatic analyses of all available USP structures and their sequence homologs revealed that these two new USP structures share overall structural similarity with structures of USPs previously determined. Clustering and cladogram analyses, however, show how they diverge from other members of the USP superfamily and show greater similarity to USPs from organisms inhabiting extreme environments. We compared them with other archaeal and bacterial USPs and discuss their similarities and differences in context of structure, sequential motifs, and potential function. We also attempted to group all analyzed USPs into families, so that assignment of the potential function to those with no experimental data available would be possible by extrapolation.

PubMed: 23745136
DOI: 10.1111/eva.12057

Experimental method

X-RAY DIFFRACTION (2.1 Å)