3QT1

RNA polymerase II variant containing A Chimeric RPB9-C11 subunit

3QT1 の概要

• エントリーDOI: 10.2210/pdb3qt1/pdb
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (14 entities in total)
• 機能のキーワード: transferase-transcription complex, rna polymerase ii, transcription, elongation, mrna cleavage, transferase
• 由来する生物種: Saccharomyces cerevisiae (yeast, yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P38902 P08518 P16370 P20433 P20434 P34087 P20436; Nucleus, nucleolus : Q04307 P22139 P40422; Cytoplasm : P20435
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 515537.26

構造登録者

Ruan, W.,Lehmann, E.,Thomm, M.,Kostrewa, D.,Cramer, P. (登録日: 2011-02-22, 公開日: 2011-03-23, 最終更新日: 2024-11-20)

主引用文献

Ruan, W.,Lehmann, E.,Thomm, M.,Kostrewa, D.,Cramer, P.
Evolution of two modes of intrinsic RNA polymerase transcript cleavage.
J.Biol.Chem., 286:18701-18707, 2011

PubMed Abstract: During gene transcription, the RNA polymerase (Pol) active center can catalyze RNA cleavage. This intrinsic cleavage activity is strong for Pol I and Pol III but very weak for Pol II. The reason for this difference is unclear because the active centers of the polymerases are virtually identical. Here we show that Pol II gains strong cleavage activity when the C-terminal zinc ribbon domain (C-ribbon) of subunit Rpb9 is replaced by its counterpart from the Pol III subunit C11. X-ray analysis shows that the C-ribbon has detached from its site on the Pol II surface and is mobile. Mutagenesis indicates that the C-ribbon transiently inserts into the Pol II pore to complement the active center. This mechanism is also used by transcription factor IIS, a factor that can bind Pol II and induce strong RNA cleavage. Together with published data, our results indicate that Pol I and Pol III contain catalytic C-ribbons that complement the active center, whereas Pol II contains a non-catalytic C-ribbon that is immobilized on the enzyme surface. Evolution of the Pol II system may have rendered mRNA transcript cleavage controllable by the dissociable factor transcription factor IIS to enable promoter-proximal gene regulation and elaborate 3'-processing and transcription termination.

PubMed: 21454497
DOI: 10.1074/jbc.M111.222273

実験手法

X-RAY DIFFRACTION (4.3 Å)