3QSQ

Crystal structure of the projection domain of the human astrovirus capsid protein

3QSQ の概要

• エントリーDOI: 10.2210/pdb3qsq/pdb
• 分子名称: Capsid polyprotein (2 entities in total)
• 機能のキーワード: receptor binding domain, astrovirus capsid, viral protein
• 由来する生物種: Human astrovirus 8 (HAstV-8)
• 細胞内の位置: Virion (Potential): Q9IFX1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27222.50

構造登録者

Dong, J.,Dong, L.,Mendez, E.,Tao, Y.J. (登録日: 2011-02-21, 公開日: 2011-07-20, 最終更新日: 2024-02-21)

主引用文献

Dong, J.,Dong, L.,Mendez, E.,Tao, Y.
Crystal structure of the human astrovirus capsid spike.
Proc.Natl.Acad.Sci.USA, 108:12681-12686, 2011

PubMed Abstract: Astroviruses are single-stranded, plus-sense RNA viruses that infect both mammals and birds, causing gastroenteritis and other extraintestinal diseases. Clinical studies have established astroviruses as the second leading cause of viral diarrhea in young children. Here we report the crystal structure of the human astrovirus dimeric surface spike determined to 1.8-Å resolution. The overall structure of each spike/projection domain has a unique three-layered β-sandwiches fold, with a core, six-stranded β-barrel structure that is also found in the hepatitis E virus capsid protrusions, suggesting a closer phylogenetic relationship between these two viruses than previously acknowledged. Based on a hepatitis E virus capsid model, we performed homology modeling and produced a complete, T = 3 astrovirus capsid model with features remarkably similar to those observed in a cryoelectron microscopy reconstruction image of a human astrovirus. Mapping conserved residues onto the astrovirus projection domain revealed a putative receptor binding site with amino acid compositions characteristic for polysaccharide recognition. Our results will have an important impact on future characterization of astrovirus structure and function, and will likely have practical applications in the development of vaccines and antivirals.

PubMed: 21768348
DOI: 10.1073/pnas.1104834108

実験手法

X-RAY DIFFRACTION (1.8 Å)