3QRN

X-ray crystal structure of the ruthenium complex [Ru(tap)2(dppz)]2+ bound to d(TCGGCGCCGA)at high resolution

3QRN の概要

• エントリーDOI: 10.2210/pdb3qrn/pdb
• 関連するPDBエントリー: 3QF8
• 分子名称: 5'-D(*TP*CP*GP*GP*CP*GP*CP*CP*GP*A)-3', Ru(tap)2(dppz) complex, BARIUM ION, ... (4 entities in total)
• 機能のキーワード: intercalation, semi-intercalation, b-dna, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3931.05

構造登録者

Cardin, C.J.,Hall, J.P. (登録日: 2011-02-18, 公開日: 2011-10-19, 最終更新日: 2023-11-01)

主引用文献

Hall, J.P.,O'Sullivan, K.,Naseer, A.,Smith, J.A.,Kelly, J.M.,Cardin, C.J.
Structure determination of an intercalating ruthenium dipyridophenazine complex which kinks DNA by semiintercalation of a tetraazaphenanthrene ligand.
Proc.Natl.Acad.Sci.USA, 108:17610-17614, 2011

PubMed Abstract: We describe a crystal structure, at atomic resolution (1.1 Å, 100 K), of a ruthenium polypyridyl complex bound to duplex DNA, in which one ligand acts as a wedge in the minor groove, resulting in the 51° kinking of the double helix. The complex cation Λ-[Ru(1,4,5,8-tetraazaphenanthrene)(2)(dipyridophenazine)](2+) crystallizes in a 11 ratio with the oligonucleotide d(TCGGCGCCGA) in the presence of barium ions. Each complex binds to one duplex by intercalation of the dipyridophenazine ligand and also by semiintercalation of one of the orthogonal tetraazaphenanthrene ligands into a second symmetrically equivalent duplex. The result is noncovalent cross-linking and marked kinking of DNA.

PubMed: 21969542
DOI: 10.1073/pnas.1108685108

実験手法

X-RAY DIFFRACTION (1.1 Å)