3QPT

Crystal structure of the Salmonella transcriptional regulator SlyA

3QPT の概要

• エントリーDOI: 10.2210/pdb3qpt/pdb
• 関連するPDBエントリー: 3Q5F
• 分子名称: Transcriptional regulator slyA (2 entities in total)
• 機能のキーワード: marr/slya protein family, winged helix-turn-helix, transcriptonal regulator, transcription
• 由来する生物種: Salmonella enterica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16902.07

構造登録者

Dolan, K.T.,Duguid, E.M. (登録日: 2011-02-14, 公開日: 2011-05-04, 最終更新日: 2024-10-30)

主引用文献

Dolan, K.T.,Duguid, E.M.,He, C.
Crystal Structures of SlyA Protein, a Master Virulence Regulator of Salmonella, in Free and DNA-bound States.
J.Biol.Chem., 286:22178-22185, 2011

PubMed Abstract: SlyA is a master virulence regulator that controls the transcription of numerous genes in Salmonella enterica. We present here crystal structures of SlyA by itself and bound to a high-affinity DNA operator sequence in the slyA gene. SlyA interacts with DNA through direct recognition of a guanine base by Arg-65, as well as interactions between conserved Arg-86 and the minor groove and a large network of non-base-specific contacts with the sugar phosphate backbone. Our structures, together with an unpublished structure of SlyA bound to the small molecule effector salicylate (Protein Data Bank code 3DEU), reveal that, unlike many other MarR family proteins, SlyA dissociates from DNA without large conformational changes when bound to this effector. We propose that SlyA and other MarR global regulators rely more on indirect readout of DNA sequence to exert control over many genes, in contrast to proteins (such as OhrR) that recognize a single operator.

PubMed: 21550983
DOI: 10.1074/jbc.M111.245258

実験手法

X-RAY DIFFRACTION (2.4 Å)