3QO7

Crystal structure of the seryl-tRNA synthetase from Candida albicans

3QO7 の概要

• エントリーDOI: 10.2210/pdb3qo7/pdb
• 関連するPDBエントリー: 3QNE 3QO5 3QO8
• 分子名称: Seryl-tRNA synthetase, cytoplasmic, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: class-ii aminoacyl-trna synthetase family, type-1 seryl-trna synthetase subfamily, aminoacyl-trna synthetase, trna, serine, amino acid biosynthesis, ctg-clade, codon ambiguity, pathogen, ligase
• 由来する生物種: Candida albicans (yeast)
• 細胞内の位置: Cytoplasm: Q9HGT6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56190.05

構造登録者

Rocha, R.,Santos, M.A.,Pereira, P.J.B.,Macedo-Ribeiro, S. (登録日: 2011-02-09, 公開日: 2011-08-03, 最終更新日: 2023-09-13)

主引用文献

Rocha, R.,Pereira, P.J.,Santos, M.A.,Macedo-Ribeiro, S.
Unveiling the structural basis for translational ambiguity tolerance in a human fungal pathogen.
Proc.Natl.Acad.Sci.USA, 108:14091-14096, 2011

PubMed Abstract: In a restricted group of opportunistic fungal pathogens the universal leucine CUG codon is translated both as serine (97%) and leucine (3%), challenging the concept that translational ambiguity has a negative impact in living organisms. To elucidate the molecular mechanisms underlying the in vivo tolerance to a nonconserved genetic code alteration, we have undertaken an extensive structural analysis of proteins containing CUG-encoded residues and solved the crystal structures of the two natural isoforms of Candida albicans seryl-tRNA synthetase. We show that codon reassignment resulted in a nonrandom genome-wide CUG redistribution tailored to minimize protein misfolding events induced by the large-scale leucine-to-serine replacement within the CTG clade. Leucine or serine incorporation at the CUG position in C. albicans seryl-tRNA synthetase induces only local structural changes and, although both isoforms display tRNA serylation activity, the leucine-containing isoform is more active. Similarly, codon ambiguity is predicted to shape the function of C. albicans proteins containing CUG-encoded residues in functionally relevant positions, some of which have a key role in signaling cascades associated with morphological changes and pathogenesis. This study provides a first detailed analysis on natural reassignment of codon identity, unveiling a highly dynamic evolutionary pattern of thousands of fungal CUG codons to confer an optimized balance between protein structural robustness and functional plasticity.

PubMed: 21825144
DOI: 10.1073/pnas.1102835108

実験手法

X-RAY DIFFRACTION (2.55 Å)