3QL2
Crystal Structure of Ribonuclease A Variant A4C/D83E/V118C
Summary for 3QL2
| Entry DOI | 10.2210/pdb3ql2/pdb |
| Related | 3QL1 |
| Descriptor | Ribonuclease pancreatic, SULFATE ION (3 entities in total) |
| Functional Keywords | ribonuclease a, disulfide bond, salt bridge, stability, folding, proteolysis, site-directed mutagenesis, hydrolase |
| Biological source | Bos taurus (bovine) |
| Cellular location | Secreted: P61823 |
| Total number of polymer chains | 2 |
| Total formula weight | 27708.99 |
| Authors | Arnold, U.,Schoepfel, M. (deposition date: 2011-02-02, release date: 2012-02-29, Last modification date: 2024-10-30) |
| Primary citation | Arnold, U.,Schopfel, M. Significant stabilization of ribonuclease A by additive effects Febs J., 279:2508-2519, 2012 Cited by PubMed Abstract: Among the strategies that employ genetic engineering to stabilize proteins, the introduction of disulfide bonds has proven to be a very potential approach. As, however, the replacement of amino acid residues by cysteines and the subsequent formation of the covalent bond can result in a severe deformation of the parental protein structure, the stabilization effect is strongly context dependent. Alternatively, the introduction of charged amino acid residues at the surface, which may result in the formation of extra ionic interactions or hydrogen bonds, provide propitious means for protein stabilization. The generation of an extra disulfide bond between residues 4 and 118 in ribonuclease A had resulted in a stabilization by 6 °C or 7 kJ mol(-1), which was mainly caused by a deceleration of the unfolding reaction [Pecher, P. & Arnold, U. (2009) Biophys Chem, 141, 21-28]. Here, Asp83 was replaced by Glu resulting in a comparable stabilization. Moreover, combination of both mutations led to an additive effect and the resulting ribonuclease A variant (T(m) ~ 76 °C, ΔG° ~ 53 kJ mol(-1)) is the most stable ribonuclease A variant described so far. The analysis of the crystal structure of A4C/D83E/V118C-ribonuclease A reveals the formation of a salt bridge between the γ-carboxyl group of Glu83 and the ε-amino group of Lys104. PubMed: 22594773DOI: 10.1111/j.1742-4658.2012.08632.x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.49 Å) |
Structure validation
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