3QKR

Mre11 Rad50 binding domain bound to Rad50

3QKR の概要

• エントリーDOI: 10.2210/pdb3qkr/pdb
• 関連するPDBエントリー: 3QKS 3QKT 3QKU
• 分子名称: DNA double-strand break repair rad50 ATPase, DNA double-strand break repair protein mre11, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: reca-like fold, coiled-coils, atpase, exonuclease, endonuclease, atp binding, dna binding, replication
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 48421.53

構造登録者

Williams, G.J.,Williams, R.S.,Arvai, A.,Moncalian, G.,Tainer, J.A. (登録日: 2011-02-01, 公開日: 2011-03-30, 最終更新日: 2023-09-13)

主引用文献

Williams, G.J.,Williams, R.S.,Williams, J.S.,Moncalian, G.,Arvai, A.S.,Limbo, O.,Guenther, G.,Sildas, S.,Hammel, M.,Russell, P.,Tainer, J.A.
ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.
Nat.Struct.Mol.Biol., 18:423-431, 2011

PubMed Abstract: The Rad50 ABC-ATPase complex with Mre11 nuclease is essential for dsDNA break repair, telomere maintenance and ataxia telangiectasia-mutated kinase checkpoint signaling. How Rad50 affects Mre11 functions and how ABC-ATPases communicate nucleotide binding and ligand states across long distances and among protein partners are questions that have remained obscure. Here, structures of Mre11-Rad50 complexes define the Mre11 2-helix Rad50 binding domain (RBD) that forms a four-helix interface with Rad50 coiled coils adjoining the ATPase core. Newly identified effector and basic-switch helix motifs extend the ABC-ATPase signature motif to link ATP-driven Rad50 movements to coiled coils binding Mre11, implying an ~30-Å pull on the linker to the nuclease domain. Both RBD and basic-switch mutations cause clastogen sensitivity. Our new results characterize flexible ATP-dependent Mre11 regulation, defects in cancer-linked RBD mutations, conserved superfamily basic switches and motifs effecting ATP-driven conformational change, and they provide a unified comprehension of ABC-ATPase activities.

PubMed: 21441914
DOI: 10.1038/nsmb.2038

実験手法

X-RAY DIFFRACTION (3.4 Å)