3QG9

crystal structure of FBF-2/gld-1 FBEa A7U mutant complex

3QG9 の概要

• エントリーDOI: 10.2210/pdb3qg9/pdb
• 関連するPDBエントリー: 3K5Y 3QGB 3QGC
• 分子名称: Fem-3 mRNA-binding factor 2, 5'-R(*UP*GP*UP*GP*CP*CP*UP*UP*A)-3', 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: puf repeats, rna binding domain, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Caenorhabditis elegans (nematode); 詳細
• 細胞内の位置: Cytoplasm : Q09312
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49890.81

構造登録者

Koh, Y.Y.,Wang, Y.,Qiu, C.,Opperman, L.,Gross, L.,Hall, T.M.T.,Wickens, M. (登録日: 2011-01-24, 公開日: 2011-03-23, 最終更新日: 2024-11-06)

主引用文献

Koh, Y.Y.,Wang, Y.,Qiu, C.,Opperman, L.,Gross, L.,Tanaka Hall, T.M.,Wickens, M.
Stacking interactions in PUF-RNA complexes.
Rna, 17:718-727, 2011

PubMed Abstract: Stacking interactions between amino acids and bases are common in RNA-protein interactions. Many proteins that regulate mRNAs interact with single-stranded RNA elements in the 3' UTR (3'-untranslated region) of their targets. PUF proteins are exemplary. Here we focus on complexes formed between a Caenorhabditis elegans PUF protein, FBF, and its cognate RNAs. Stacking interactions are particularly prominent and involve every RNA base in the recognition element. To assess the contribution of stacking interactions to formation of the RNA-protein complex, we combine in vivo selection experiments with site-directed mutagenesis, biochemistry, and structural analysis. Our results reveal that the identities of stacking amino acids in FBF affect both the affinity and specificity of the RNA-protein interaction. Substitutions in amino acid side chains can restrict or broaden RNA specificity. We conclude that the identities of stacking residues are important in achieving the natural specificities of PUF proteins. Similarly, in PUF proteins engineered to bind new RNA sequences, the identity of stacking residues may contribute to "target" versus "off-target" interactions, and thus be an important consideration in the design of proteins with new specificities.

PubMed: 21372189
DOI: 10.1261/rna.2540311

実験手法

X-RAY DIFFRACTION (2.25 Å)