3QE6

Mouse PACSIN 3 F-BAR domain structure

3QE6 の概要

• エントリーDOI: 10.2210/pdb3qe6/pdb
• 分子名称: Protein kinase C and casein kinase II substrate protein 3, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: f-bar domain, endocytosis
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Cytoplasm: Q99JB8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71314.35

構造登録者

Meng, G.,Bai, X.,Zheng, X. (登録日: 2011-01-19, 公開日: 2012-01-25, 最終更新日: 2023-11-01)

主引用文献

Bai, X.,Meng, G.,Luo, M.,Zheng, X.
Rigidity of wedge loop in PACSIN 3 protein is a key factor in dictating diameters of tubules
J.Biol.Chem., 287:22387-22396, 2012

PubMed Abstract: BAR (Bin/amphiphysin/Rvs) domain-containing proteins participate in cellular membrane remodeling. The F-BAR proteins normally generate low curvature tubules. However, in the PACSIN subfamily, the F-BAR domain from PACSIN 1 and 2 can induce both high and low curvature tubules. We found that unlike PACSIN 1 and 2, PACSIN 3 could only induce low curvature tubules. To elucidate the key factors that dictate the tubule curvature, crystal structures of all three PACSIN F-BAR domains were determined. A novel type of lateral interaction mediated by a wedge loop is observed between the F-BAR neighboring dimers. Comparisons of the structures of PACSIN 3 with PACSIN 1 and 2 indicate that the wedge loop of PACSIN 3 is more rigid, which influences the lateral interactions between assembled dimers. We further identified the residues that affect the rigidity of the loop by mutagenesis and determined the structures of two PACSIN 3 wedge loop mutants. Our results suggest that the rigidity-mediated conformations of the wedge loop correlate well with the various crystal packing modes and membrane tubulations. Thus, the rigidity of the wedge loop is a key factor in dictating tubule diameters.

PubMed: 22573331
DOI: 10.1074/jbc.M112.358960

実験手法

X-RAY DIFFRACTION (2.6 Å)