3QDS

Structure of apo Boletus edulis lectin

3QDS の概要

• エントリーDOI: 10.2210/pdb3qds/pdb
• 関連するPDBエントリー: 3QDT 3QDU 3QDV 3QDW 3QDX 3QDY
• 分子名称: BOLETUS EDULIS LECTIN (2 entities in total)
• 機能のキーワード: boletus edulis, lectin, mushroom, t-antigen, carbohydrate, sugar binding, sugar binding protein
• 由来する生物種: Boletus edulis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31703.67

構造登録者

Bovi, M.,Carrizo, M.E.,Capaldi, S.,Perduca, M.,Chiarelli, L.R.,Galliano, M.,Monaco, H.L. (登録日: 2011-01-19, 公開日: 2011-02-23, 最終更新日: 2024-11-20)

主引用文献

Bovi, M.,Carrizo, M.E.,Capaldi, S.,Perduca, M.,Chiarelli, L.R.,Galliano, M.,Monaco, H.L.
Structure of a lectin with antitumoral properties in king bolete (Boletus edulis) mushrooms.
Glycobiology, 21:1000-1009, 2011

PubMed Abstract: A novel lectin has been isolated from the fruiting bodies of the common edible mushroom Boletus edulis (king bolete, penny bun, porcino or cep) by affinity chromatography on a chitin column. We propose for the lectin the name BEL (B. edulis lectin). BEL inhibits selectively the proliferation of several malignant cell lines and binds the neoplastic cell-specific T-antigen disaccharide, Galβ1-3GalNAc. The lectin was structurally characterized: the molecule is a homotetramer and the 142-amino acid sequence of the chains was determined. The protein belongs to the saline-soluble family of mushroom fruiting body-specific lectins. BEL was also crystallized and its three-dimensional structure was determined by X-ray diffraction to 1.15 Å resolution. The structure is similar to that of Agaricus bisporus lectin. Using the appropriate co-crystals, the interactions of BEL with specific mono- and disaccharides were also studied by X-ray diffraction. The six structures of carbohydrate complexes reported here provide details of the interactions of the ligands with the lectin and shed light on the selectivity of the two distinct binding sites present in each protomer.

PubMed: 21303815
DOI: 10.1093/glycob/cwr012

実験手法

X-RAY DIFFRACTION (1.15 Å)