3QCA

Crystal Structure of FAF1 UBX Domain In Complex with p97/VCP N Domain Reveals The Conserved FcisP Touch-Turn Motif of UBX Domain Suffering Conformational Change

3QCA の概要

• エントリーDOI: 10.2210/pdb3qca/pdb
• 関連するPDBエントリー: 3QC8
• 分子名称: FAS-associated factor 1 (2 entities in total)
• 機能のキーワード: ubx, faf1, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Potential): Q9UNN5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 39341.00

構造登録者

Kim, K.H.,Kang, W.,Suh, S.W.,Yang, J.K. (登録日: 2011-01-15, 公開日: 2011-05-25, 最終更新日: 2024-03-20)

主引用文献

Kang, W.,Yang, J.K.
Crystal structure of human FAF1 UBX domain reveals a novel FcisP touch-turn motif in p97/VCP-binding region
Biochem.Biophys.Res.Commun., 407:531-534, 2011

PubMed Abstract: UBX domain is a general p97/VCP-binding module found in an increasing number of proteins including FAF1, p47, SAKS1 and UBXD7. FAF1, a multi-functional tumor suppressor protein, binds to the N domain of p97/VCP through its C-terminal UBX domain and thereby inhibits the proteasomal protein degradation in which p97/VCP acts as a co-chaperone. Here we report the crystal structure of human FAF1 UBX domain at 2.9Å resolution. It reveals that the conserved FP sequence in the p97/VCP-binding region adopts a rarely observed cis-Pro touch-turn structure. We call it an FcisP touch-turn motif and suggest that it is the conserved structural element of the UBX domain. Four FAF1 UBX molecules in an asymmetric unit of the crystal show two different conformations of the FcisP touch-turn motif. The phenyl ring of F(619) in the motif stacks partly over cis-Pro(620) in one conformation, whereas it is swung out from cis-P(620), in the other conformation, and forms hydrophobic contacts with the residues of the neighboring molecule. In addition, the entire FcisP touch-turn motif is pulled out in the second conformation by about 2Å in comparison to the first conformation. Those conformational differences observed in the p97/VCP-binding motif caused by the interaction with neighboring molecules presumably represent the conformational change of the UBX domain on its binding to the N domain of p97/VCP.

PubMed: 21414298
DOI: 10.1016/j.bbrc.2011.03.052

実験手法

X-RAY DIFFRACTION (2.9 Å)