3QB2

The Crystal Structure of Immunity Factor for SPN (IFS)

3QB2 の概要

• エントリーDOI: 10.2210/pdb3qb2/pdb
• 関連するPDBエントリー: 3PNT
• 分子名称: Immunity factor for SPN, SULFATE ION (3 entities in total)
• 機能のキーワード: glycohydrolase inhibitor, streptococcus pyogenes glycohydrolase toxin, hydrolase inhibitor
• 由来する生物種: Streptococcus pyogenes
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 90036.76

構造登録者

Smith, C.L.,Ellenberger, T. (登録日: 2011-01-12, 公開日: 2011-03-16, 最終更新日: 2017-11-08)

主引用文献

Smith, C.L.,Ghosh, J.,Elam, J.S.,Pinkner, J.S.,Hultgren, S.J.,Caparon, M.G.,Ellenberger, T.
Structural Basis of Streptococcus pyogenes Immunity to Its NAD(+) Glycohydrolase Toxin.
Structure, 19:192-202, 2011

PubMed Abstract: The virulence of Gram-positive bacteria is enhanced by toxins like the Streptococcus pyogenes β-NAD(+) glycohydrolase known as SPN. SPN-producing strains of S. pyogenes additionally express the protein immunity factor for SPN (IFS), which forms an inhibitory complex with SPN. We have determined crystal structures of the SPN-IFS complex and IFS alone, revealing that SPN is structurally related to ADP-ribosyl transferases but lacks the canonical binding site for protein substrates. SPN is instead a highly efficient glycohydrolase with the potential to deplete cellular levels of β-NAD(+). The protective effect of IFS involves an extensive interaction with the SPN active site that blocks access to β-NAD(+). The conformation of IFS changes upon binding to SPN, with repacking of an extended C-terminal α helix into a compact shape. IFS is an attractive target for the development of novel bacteriocidal compounds functioning by blocking the bacterium's self-immunity to the SPN toxin.

PubMed: 21300288
DOI: 10.1016/j.str.2010.12.013

実験手法

X-RAY DIFFRACTION (2.5 Å)