3Q4S

Crystal Structure of Human Glycogenin-1 (GYG1), apo form

3Q4S の概要

• エントリーDOI: 10.2210/pdb3q4s/pdb
• 分子名称: Glycogenin-1, 1,2-ETHANEDIOL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: structural genomics consortium, sgc, glycosyltransferase, glycogen biosynthesis, glycosylation, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30189.70

構造登録者

Chaikuad, A.,Froese, D.S.,Yue, W.W.,Krysztofinska, E.,von Delft, F.,Weigelt, J.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,Oppermann, O.,Structural Genomics Consortium (SGC) (登録日: 2010-12-24, 公開日: 2011-02-09, 最終更新日: 2023-09-13)

主引用文献

Chaikuad, A.,Froese, D.S.,Berridge, G.,von Delft, F.,Oppermann, U.,Yue, W.W.
Conformational plasticity of glycogenin and its maltosaccharide substrate during glycogen biogenesis.
Proc.Natl.Acad.Sci.USA, 108:21028-21033, 2011

PubMed Abstract: Glycogenin initiates the synthesis of a maltosaccharide chain covalently attached to itself on Tyr195 via a stepwise glucosylation reaction, priming glycogen synthesis. We have captured crystallographic snapshots of human glycogenin during its reaction cycle, revealing a dynamic conformational switch between ground and active states mediated by the sugar donor UDP-glucose. This switch includes the ordering of a polypeptide stretch containing Tyr195, and major movement of an approximately 30-residue "lid" segment covering the active site. The rearranged lid guides the nascent maltosaccharide chain into the active site in either an intra- or intersubunit mode dependent upon chain length and steric factors and positions the donor and acceptor sugar groups for catalysis. The Thr83Met mutation, which causes glycogen storage disease XV, is conformationally locked in the ground state and catalytically inactive. Our data highlight the conformational plasticity of glycogenin and coexistence of two modes of glucosylation as integral to its catalytic mechanism.

PubMed: 22160680
DOI: 10.1073/pnas.1113921108

実験手法

X-RAY DIFFRACTION (1.98 Å)