3PX8

RTA in complex with 7-carboxy-pterin

3PX8 の概要

• エントリーDOI: 10.2210/pdb3px8/pdb
• 関連するPDBエントリー: 3PX9
• 分子名称: Preproricin, 2-amino-4-oxo-1,4-dihydropteridine-7-carboxylic acid (3 entities in total)
• 機能のキーワード: ricin, toxin, protein-ligand complex, hydrolase, ribosome inactivating protein, n-glycosidase, pterin, hydrolase-inhibitor complex, hydrolase/inhibitor
• 由来する生物種: Ricinus communis (Castor bean)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29142.77

構造登録者

Jasheway, K.R.,Robertus, J.D. (登録日: 2010-12-09, 公開日: 2011-06-22, 最終更新日: 2024-02-21)

主引用文献

Pruet, J.M.,Jasheway, K.R.,Manzano, L.A.,Bai, Y.,Anslyn, E.V.,Robertus, J.D.
7-Substituted pterins provide a new direction for ricin A chain inhibitors.
Eur.J.Med.Chem., 46:3608-3615, 2011

PubMed Abstract: Ricin is a potent toxin found in castor seeds. The A chain, RTA, enzymaticlly depurinates a specific adenosine in ribosomal RNA, inhibiting protein synthesis. Ricin is a known chemical weapons threat having no effective antidote. This makes the discovery of new inhibitors of great importance. We have previously used 6-substituted pterins, such as pteroic acid, as an inhibitor platform with moderate success. We now report the success of 7-carboxy pterin (7CP) as an RTA inhibitor; its binding has been monitored using both kinetic and temperature shift assays and by X-ray crystallography. We also discuss the synthesis of various derivatives of 7CP, and their binding affinity and inhibitory effects, as part of a program to make effective RTA inhibitors.

PubMed: 21641093
DOI: 10.1016/j.ejmech.2011.05.025

実験手法

X-RAY DIFFRACTION (1.29 Å)