3PSA

Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to tungstate)

3PSA の概要

• エントリーDOI: 10.2210/pdb3psa/pdb
• 関連するPDBエントリー: 3PSH
• 分子名称: protein HI_1472, TUNGSTATE(VI)ION (3 entities in total)
• 機能のキーワード: periplasmic binding protein, substrate binding protein, oxyanion binding protein, metal transport
• 由来する生物種: Haemophilus influenzae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36890.77

構造登録者

Tirado-Lee, L.,Lee, A.,Rees, D.C.,Pinkett, H.W. (登録日: 2010-12-01, 公開日: 2011-11-30, 最終更新日: 2024-02-21)

主引用文献

Tirado-Lee, L.,Lee, A.,Rees, D.C.,Pinkett, H.W.
Classification of a Haemophilus influenzae ABC Transporter HI1470/71 through Its Cognate Molybdate Periplasmic Binding Protein, MolA.
Structure, 19:1701-1710, 2011

PubMed Abstract: molA (HI1472) from H. influenzae encodes a periplasmic binding protein (PBP) that delivers substrate to the ABC transporter MolB(2)C(2) (formerly HI1470/71). The structures of MolA with molybdate and tungstate in the binding pocket were solved to 1.6 and 1.7 Å resolution, respectively. The MolA-binding protein binds molybdate and tungstate, but not other oxyanions such as sulfate and phosphate, making it the first class III molybdate-binding protein structurally solved. The ∼100 μM binding affinity for tungstate and molybdate is significantly lower than observed for the class II ModA molybdate-binding proteins that have nanomolar to low micromolar affinity for molybdate. The presence of two molybdate loci in H. influenzae suggests multiple transport systems for one substrate, with molABC constituting a low-affinity molybdate locus.

PubMed: 22078568
DOI: 10.1016/j.str.2011.10.004

実験手法

X-RAY DIFFRACTION (1.7 Å)