3POV

Crystal structure of a SOX-DNA complex

3POV の概要

• エントリーDOI: 10.2210/pdb3pov/pdb
• 分子名称: ORF 37, DNA (5'-D(*GP*GP*GP*AP*TP*CP*CP*TP*CP*CP*CP*AP*GP*TP*CP*GP*AP*CP*C)-3'), DNA (5'-D(*GP*GP*TP*CP*GP*AP*CP*TP*AP*GP*GP*AP*GP*GP*AP*TP*CP*CP*C)-3'), ... (6 entities in total)
• 機能のキーワード: type ii restriction endonuclease superfamily, nuclease, nucleus/cytoplasm, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Human herpesvirus 8 type M; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 67032.05

構造登録者

Bagneris, C.,Barrett, T.E. (登録日: 2010-11-23, 公開日: 2011-09-21, 最終更新日: 2023-11-01)

主引用文献

Bagneris, C.,Briggs, L.C.,Savva, R.,Ebrahimi, B.,Barrett, T.E.
Crystal structure of a KSHV-SOX-DNA complex: insights into the molecular mechanisms underlying DNase activity and host shutoff
Nucleic Acids Res., 39:5744-5756, 2011

PubMed Abstract: The early lytic phase of Kaposi's sarcoma herpesvirus infection is characterized by viral replication and the global degradation (shutoff) of host mRNA. Key to both activities is the virally encoded alkaline exonuclease KSHV SOX. While the DNase activity of KSHV SOX is required for the resolution of viral genomic DNA as a precursor to encapsidation, its exact involvement in host shutoff remains to be determined. We present the first crystal structure of a KSHV SOX-DNA complex that has illuminated the catalytic mechanism underpinning both its endo and exonuclease activities. We further illustrate that KSHV SOX, similar to its Epstein-Barr virus homologue, has an intrinsic RNase activity in vitro that although an element of host shutoff, cannot solely account for the phenomenon.

PubMed: 21421561
DOI: 10.1093/nar/gkr111

実験手法

X-RAY DIFFRACTION (2.5 Å)