3POM

Crystal Structure of the Unliganded Retinoblastoma Protein Pocket Domain

3POM の概要

• エントリーDOI: 10.2210/pdb3pom/pdb
• 分子名称: Retinoblastoma-associated protein (2 entities in total)
• 機能のキーワード: cyclin fold, tumor suppressor protein, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : P06400
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82427.85

構造登録者

Balog, E.R.M.,Burke, J.R.,Rubin, S.M. (登録日: 2010-11-23, 公開日: 2011-04-27, 最終更新日: 2024-11-06)

主引用文献

Balog, E.R.,Burke, J.R.,Hura, G.L.,Rubin, S.M.
Crystal structure of the unliganded retinoblastoma protein pocket domain.
Proteins, 79:2010-2014, 2011

PubMed Abstract: The retinoblastoma protein (Rb) regulates cell proliferation through its association with E2F transcription factors and other proteins. The Rb “pocket” domain primarily facilitates protein-protein interactions, and several structures of the pocket bound to E2F and tumorigenic viral proteins have been reported. We report here the first crystal structure of the pocket domain without bound ligand. We find that ligand association results in observable structural changes at the binding sites but no significant changes to the overall conformation of the domain. These data support models for regulation of Rb-E2F binding that do not require considerable structural changes in the pocket domain.

PubMed: 21491492
DOI: 10.1002/prot.23007

実験手法

X-RAY DIFFRACTION (2.5 Å)