3PJP

A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain(CTD)

Replaces:  3OR8

Summary for 3PJP

• Entry DOI: 10.2210/pdb3pjp/pdb
• Descriptor: Transcription elongation factor SPT6, ACETATE ION, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• Functional Keywords: sh2, transcription, transcription elongation, ctd binding, nucleus
• Biological source: Candida glabrata (yeast)
• Cellular location: Nucleus (By similarity): Q6FLB1
• Total number of polymer chains: 2
• Total formula weight: 47599.17

Authors

Sun, M.,Lariviere, L.,Dengl, S.,Mayer, A.,Cramer, P. (deposition date: 2010-11-10, release date: 2010-11-24, Last modification date: 2024-02-21)

Primary citation

Sun, M.,Lariviere, L.,Dengl, S.,Mayer, A.,Cramer, P.
A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II C-terminal repeat domain (CTD).
J.Biol.Chem., 285:41597-41603, 2010

PubMed Abstract: Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo as its deletion in cells is lethal in the presence of 6-azauracil.

PubMed: 20926372
DOI: 10.1074/jbc.M110.144568

Experimental method

X-RAY DIFFRACTION (1.6 Å)