3PJP

A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain(CTD)

「3OR8」から置き換えられました

3PJP の概要

• エントリーDOI: 10.2210/pdb3pjp/pdb
• 分子名称: Transcription elongation factor SPT6, ACETATE ION, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: sh2, transcription, transcription elongation, ctd binding, nucleus
• 由来する生物種: Candida glabrata (yeast)
• 細胞内の位置: Nucleus (By similarity): Q6FLB1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47599.17

構造登録者

Sun, M.,Lariviere, L.,Dengl, S.,Mayer, A.,Cramer, P. (登録日: 2010-11-10, 公開日: 2010-11-24, 最終更新日: 2024-02-21)

主引用文献

Sun, M.,Lariviere, L.,Dengl, S.,Mayer, A.,Cramer, P.
A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II C-terminal repeat domain (CTD).
J.Biol.Chem., 285:41597-41603, 2010

PubMed Abstract: Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo as its deletion in cells is lethal in the presence of 6-azauracil.

PubMed: 20926372
DOI: 10.1074/jbc.M110.144568

実験手法

X-RAY DIFFRACTION (1.6 Å)