3PIJ

beta-fructofuranosidase from Bifidobacterium longum - complex with fructose

Summary for 3PIJ

• Entry DOI: 10.2210/pdb3pij/pdb
• Related: 3PIG
• Descriptor: Beta-fructofuranosidase, beta-D-fructofuranose, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: five-bladed beta-propeller and beta-sandwich domains, glycoside hydrolase family 32, probiotic bacteria, fructose, hydrolase
• Biological source: Bifidobacterium longum
• Total number of polymer chains: 2
• Total formula weight: 118869.16

Authors

Bujacz, A.,Bujacz, G.,Redzynia, I.,Krzepkowska-Jedrzejczak, M.,Bielecki, S. (deposition date: 2010-11-06, release date: 2011-04-27, Last modification date: 2023-09-06)

Primary citation

Bujacz, A.,Jedrzejczak-Krzepkowska, M.,Bielecki, S.,Redzynia, I.,Bujacz, G.
Crystal structures of the apo form of beta-fructofuranosidase from Bifidobacterium longum and its complex with fructose
Febs J., 278:1728-1744, 2011

PubMed Abstract: We solved the 1.8 Å crystal structure of β-fructofuranosidase from Bifidobacterium longum KN29.1 - a unique enzyme that allows these probiotic bacteria to function in the human digestive system. The sequence of β-fructofuranosidase classifies it as belonging to the glycoside hydrolase family 32 (GH32). GH32 enzymes show a wide range of substrate specificity and different functions in various organisms. All enzymes from this family share a similar fold, containing two domains: an N-terminal five-bladed β-propeller and a C-terminal β-sandwich module. The active site is located in the centre of the β-propeller domain, in the bottom of a 'funnel'. The binding site, -1, responsible for tight fructose binding, is highly conserved among the GH32 enzymes. Bifidobacterium longum KN29.1 β-fructofuranosidase has a 35-residue elongation of the N-terminus containing a five-turn α-helix, which distinguishes it from the other known members of the GH32 family. This new structural element could be one of the functional modifications of the enzyme that allows the bacteria to act in a human digestive system. We also solved the 1.8 Å crystal structure of the β-fructofuranosidase complex with β-D-fructose, a hydrolysis product obtained by soaking apo crystal in raffinose.

PubMed: 21418142
DOI: 10.1111/j.1742-4658.2011.08098.x

Experimental method

X-RAY DIFFRACTION (1.8 Å)