3PIC

Glucuronoyl Esterase catalytic domain (Cip2_GE) from Hypocrea jecorina

3PIC の概要

• エントリーDOI: 10.2210/pdb3pic/pdb
• 分子名称: Cip2, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: alpha/beta hydrolase fold, glucuronoyl esterase, carbohydrate esterase family 15 (ce-15), n-linked glycosylation, secreted, hydrolase
• 由来する生物種: Hypocrea jecorina (Trichoderma reesei)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 119806.69

構造登録者

Pokkuluri, P.R. (登録日: 2010-11-05, 公開日: 2011-06-01, 最終更新日: 2024-10-30)

主引用文献

Pokkuluri, P.R.,Duke, N.E.,Wood, S.J.,Cotta, M.A.,Li, X.L.,Biely, P.,Schiffer, M.
Structure of the catalytic domain of glucuronoyl esterase Cip2 from Hypocrea jecorina.
Proteins, 79:2588-2592, 2011

PubMed Abstract: The structure of the catalytic domain of glucuronoyl esterase Cip2 from the fungus H. jecorina was determined at a resolution of 1.9 Å. This is the first structure of the newly established carbohydrate esterase family 15. The structure has revealed the residues Ser278-His411-Glu301 present in a triad arrangement as the active site. Ser278 is present in the novel consensus sequence GCSRXG reported earlier in the members of CE-15 family. The active site is exposed on the surface of the protein which has implications for the ability of the enzyme to hydrolyze ester bonds of large substrates. Efforts are underway to obtain crystals of Cip2_GE complexed with inhibitor and synthetic substrates. The activity of the glucuronoyl esterase could play a significant role in plant biomass degradation as its expected role is to separate the lignin from hemicelluloses by hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid moieties of glucuronoxylans and aromatic alcohols of lignin.

PubMed: 21661060
DOI: 10.1002/prot.23088

実験手法

X-RAY DIFFRACTION (1.9 Å)