3PHP

STRUCTURE OF THE 3' HAIRPIN OF THE TYMV PSEUDOKNOT: PREFORMATION IN RNA FOLDING

3PHP の概要

• エントリーDOI: 10.2210/pdb3php/pdb
• NMR情報: BMRB: 4250
• 分子名称: RNA (5'-R(*GP*GP*UP*UP*CP*CP*GP*AP*GP*GP*GP*UP*CP*AP*UP*CP*GP*GP*AP*AP*CP*CP*A) -3') (1 entity in total)
• 機能のキーワード: rna, pseudoknot, tymv, ribonucleic acid
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7418.47

構造登録者

Kolk, M.H.,Van Der Graaf, M.,Wijmenga, S.S.,Pleij, C.W.A.,Heus, H.A.,Hilbers, C.W. (登録日: 1998-10-13, 公開日: 1998-10-21, 最終更新日: 2023-12-27)

主引用文献

Kolk, M.H.,van der Graaf, M.,Fransen, C.T.,Wijmenga, S.S.,Pleij, C.W.,Heus, H.A.,Hilbers, C.W.
Structure of the 3'-hairpin of the TYMV pseudoknot: preformation in RNA folding.
EMBO J., 17:7498-7504, 1998

PubMed Abstract: The solution structure of an RNA-hairpin present in the pseudoknot, which is found at the 3'-terminus of turnip yellow mosaic virus genomic RNA, has been solved by nuclear magnetic resonance spectroscopy. The loop, which contains the sequence 5'-GGGUCA-3', was found to be highly structured and, contrary to expectations, does not attain its stability through GA or GC base pair formation but by triple interactions between the tilted adenosine and the minor groove sides of the first two guanosines. Interestingly, a very similar conformation was found for the cognate pseudoknot, implying that the 3'-hairpin is preformed for folding into a pseudoknotted structure. These findings suggest a mechanism of 'predetermined-fit' as a principle in RNA folding.

PubMed: 9857204
DOI: 10.1093/emboj/17.24.7498

実験手法

SOLUTION NMR