3PHF

Crystal Structure of the Epstein-Barr virus gH and gL complex

3PHF の概要

• エントリーDOI: 10.2210/pdb3phf/pdb
• 関連するPDBエントリー: 3M1C
• 分子名称: Envelope glycoprotein H, Envelope glycoprotein L (2 entities in total)
• 機能のキーワード: virus entry, membrane fusion, glycoprotein, viral protein
• 由来する生物種: Human herpesvirus 4 (Epstein-Barr virus); 詳細
• 細胞内の位置: Virion membrane; Single-pass membrane protein (Potential): P03231; Virion membrane; Peripheral membrane protein; Extracellular side (By similarity): P03212
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1347944.26

構造登録者

Matsuura, H.,Kirschner, A.N.,Jardetzky, T.S. (登録日: 2010-11-04, 公開日: 2011-01-12, 最終更新日: 2024-11-27)

主引用文献

Matsuura, H.,Kirschner, A.N.,Longnecker, R.,Jardetzky, T.S.
Crystal structure of the Epstein-Barr virus (EBV) glycoprotein H/glycoprotein L (gH/gL) complex.
Proc.Natl.Acad.Sci.USA, 107:22641-22646, 2010

PubMed Abstract: The Epstein-Barr virus (EBV) is a γ-herpesvirus that infects B cells and epithelial cells and that has been linked to malignancies in both cell types in vivo. EBV, like other herpesviruses, has three glycoproteins, glycoprotein B (gB), gH, and gL, that form the core membrane fusion machinery mediating viral penetration into the cell. The gH and gL proteins associate to form a heterodimeric complex, which is necessary for efficient membrane fusion and also implicated in direct binding to epithelial cell receptors required for viral entry. To gain insight into the mechanistic role of gH/gL, we determined the crystal structure of the EBV gH/gL complex. The structure is comprised of four domains organized along the longest axis of the molecule. Comparisons with homologous HSV-2 gH/gL and partial pseudorabies virus gH structures support the domain boundaries determined for the EBV gH/gL structure and illustrate significant differences in interdomain packing angles. The gL subunit and N-terminal residues of gH form a globular domain at one end of the structure, implicated in interactions with gB and activation of membrane fusion. The C-terminal domain of gH, proximal to the viral membrane, is also implicated in membrane fusion. The gH/gL structure locates an integrin binding motif, implicated in epithelial cell entry, on a prominent loop in the central region of the structure. Multiple regions of gH/gL, including its two extreme ends, are functionally important, consistent with the multiple roles of gH/gL in EBV entry.

PubMed: 21149717
DOI: 10.1073/pnas.1011806108

実験手法

X-RAY DIFFRACTION (3.58 Å)