3PGC

Crystal Structure of HLA-DR1 with CLIP106-120, flipped peptide orientation

3PGC の概要

• エントリーDOI: 10.2210/pdb3pgc/pdb
• 関連するPDBエントリー: 3PDO 3PGD
• 分子名称: HLA class II histocompatibility antigen, DR alpha chain, HLA class II histocompatibility antigen, DRB1-1 beta chain, HLA class II histocompatibility antigen gamma chain, ... (6 entities in total)
• 機能のキーワード: mhc class ii, mhc ii, immune system, self antigen, invariant chain, clip
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: P01903 P04229; Cell membrane; Single-pass type II membrane protein (Potential): P04233
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94548.77

構造登録者

Gunther, S.,Schlundt, A.,Sticht, J.,Roske, Y.,Heinemann, U.,Wiesmuller, K.-H.,Jung, G.,Falk, K.,Rotzschke, O.,Freund, C. (登録日: 2010-11-01, 公開日: 2010-12-08, 最終更新日: 2024-11-27)

主引用文献

Gunther, S.,Schlundt, A.,Sticht, J.,Roske, Y.,Heinemann, U.,Wiesmuller, K.H.,Jung, G.,Falk, K.,Rotzschke, O.,Freund, C.
Bidirectional binding of invariant chain peptides to an MHC class II molecule.
Proc.Natl.Acad.Sci.USA, 107:22219-22224, 2010

PubMed Abstract: T-cell recognition of peptides bound to MHC class II (MHCII) molecules is a central event in cell-mediated adaptive immunity. The current paradigm holds that prebound class II-associated invariant chain peptides (CLIP) and all subsequent antigens maintain a canonical orientation in the MHCII binding groove. Here we provide evidence for MHCII-bound CLIP inversion. NMR spectroscopy demonstrates that the interconversion from the canonical to the inverse alignment is a dynamic process, and X-ray crystallography shows that conserved MHC residues form a hydrogen bond network with the peptide backbone in both orientations. The natural catalyst HLA-DM accelerates peptide reorientation and the exchange of either canonically or inversely bound CLIP against antigenic peptide. Thus, noncanonical MHC-CLIP displays the hallmarks of a structurally and functionally intact antigen-presenting complex.

PubMed: 21115828
DOI: 10.1073/pnas.1014708107

実験手法

X-RAY DIFFRACTION (2.66 Å)