3PDY

Structure of the third and fourth spectrin repeats of the plakin domain of plectin

3PDY の概要

• エントリーDOI: 10.2210/pdb3pdy/pdb
• 関連するPDBエントリー: 2IAK 2ODU 2ODV 3PE0
• 分子名称: Plectin, 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: cytoskeleton, plakin, intermediate filament, spectrin repeat, structural protein, crosslinking
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm, cytoskeleton: Q15149
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48636.61

構造登録者

Ortega, E.,de Pereda, J.M. (登録日: 2010-10-25, 公開日: 2011-02-02, 最終更新日: 2023-09-06)

主引用文献

Ortega, E.,Buey, R.M.,Sonnenberg, A.,de Pereda, J.M.
The Structure of the Plakin Domain of Plectin Reveals a Non-canonical SH3 Domain Interacting with Its Fourth Spectrin Repeat.
J.Biol.Chem., 286:12429-12438, 2011

PubMed Abstract: Plectin belongs to the plakin family of cytoskeletal crosslinkers, which is part of the spectrin superfamily. Plakins contain an N-terminal conserved region, the plakin domain, which is formed by an array of spectrin repeats (SR) and a Src-homology 3 (SH3), and harbors binding sites for junctional proteins. We have combined x-ray crystallography and small angle x-ray scattering (SAXS) to elucidate the structure of the central region of the plakin domain of plectin, which corresponds to the SR3, SR4, SR5, and SH3 domains. The crystal structures of the SR3-SR4 and SR4-SR5-SH3 fragments were determined to 2.2 and 2.95 Å resolution, respectively. The SH3 of plectin presents major alterations as compared with canonical Pro-rich binding SH3 domains, suggesting that plectin does not recognize Pro-rich motifs. In addition, the SH3 binding site is partially occluded by an intramolecular contact with the SR4. Residues of this pseudo-binding site and the SR4/SH3 interface are conserved within the plakin family, suggesting that the structure of this part of the plectin molecule is similar to that of other plakins. We have created a model for the SR3-SR4-SR5-SH3 region, which agrees well with SAXS data in solution. The three SRs form a semi-flexible rod that is not altered by the presence of the SH3 domain, and it is similar to those found in spectrins. The flexibility of the plakin domain, in analogy with spectrins, might contribute to the role of plakins in maintaining the stability of tissues subject to mechanical stress.

PubMed: 21288893
DOI: 10.1074/jbc.M110.197467

実験手法

X-RAY DIFFRACTION (2.2182 Å)