3PDI

Precursor bound NifEN

3PDI の概要

• エントリーDOI: 10.2210/pdb3pdi/pdb
• 分子名称: Nitrogenase MoFe cofactor biosynthesis protein NifE, Nitrogenase MoFe cofactor biosynthesis protein NifN, IRON/SULFUR CLUSTER, ... (4 entities in total)
• 機能のキーワード: nitrogenase cofactor maturation, nifb, nifdk, nifh, protein binding
• 由来する生物種: Azotobacter vinelandii; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 413765.73

構造登録者

Kaiser, J.T.,Hu, Y.,Wiig, J.A.,Rees, D.C.,Ribbe, M.W. (登録日: 2010-10-22, 公開日: 2011-01-19, 最終更新日: 2024-02-21)

主引用文献

Kaiser, J.T.,Hu, Y.,Wiig, J.A.,Rees, D.C.,Ribbe, M.W.
Structure of Precursor-Bound NifEN: A Nitrogenase FeMo Cofactor Maturase/Insertase.
Science, 331:91-94, 2011

PubMed Abstract: NifEN plays an essential role in the biosynthesis of the nitrogenase iron-molybdenum (FeMo) cofactor (M cluster). It is an α(2)β(2) tetramer that is homologous to the catalytic molybdenum-iron (MoFe) protein (NifDK) component of nitrogenase. NifEN serves as a scaffold for the conversion of an iron-only precursor to a matured form of the M cluster before delivering the latter to its target location within NifDK. Here, we present the structure of the precursor-bound NifEN of Azotobacter vinelandii at 2.6 angstrom resolution. From a structural comparison of NifEN with des-M-cluster NifDK and holo NifDK, we propose similar pathways of cluster insertion for the homologous NifEN and NifDK proteins.

PubMed: 21212358
DOI: 10.1126/science.1196954

実験手法

X-RAY DIFFRACTION (2.4 Å)