3P9Q

Structure of I274C variant of E. coli KatE

3P9Q の概要

• エントリーDOI: 10.2210/pdb3p9q/pdb
• 関連するPDBエントリー: 3P9P 3P9R 3P9S
• 分子名称: Catalase HPII, CIS-HEME D HYDROXYCHLORIN GAMMA-SPIROLACTONE, CIS-HEME D HYDROXYCHLORIN GAMMA-SPIROLACTONE 17R, 18S, ... (5 entities in total)
• 機能のキーワード: catalase, i274c variant, heme orientation, oxidoreductase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm : P21179
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 342009.63

構造登録者

Loewen, P.C.,Jha, V.,Louis, S.,Chelikani, P.,Carpena, X.,Fita, I. (登録日: 2010-10-18, 公開日: 2010-12-22, 最終更新日: 2024-10-30)

主引用文献

Jha, V.,Louis, S.,Chelikani, P.,Carpena, X.,Donald, L.J.,Fita, I.,Loewen, P.C.
Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli.
Biochemistry, 50:2101-2110, 2011

PubMed Abstract: Heme-containing catalases have been extensively studied, revealing the roles of many residues, the existence of two heme orientations, flipped 180° relative to one another along the propionate-vinyl axis, and the presence of both heme b and heme d. The focus of this report is a residue, situated adjacent to the vinyl groups of the heme at the entrance of the lateral channel, with an unusual main chain geometry that is conserved in all catalase structures so far determined. In Escherichia coli catalase HPII, the residue is Ile274, and replacing it with Gly, Ala, and Val, found at the same location in other catalases, results in a reduction in catalytic efficiency, a reduced intensity of the Soret absorbance band, and a mixture of heme orientations and species. The reduced turnover rates and higher H(2)O(2) concentrations required to attain equivalent reaction velocities are explained in terms of less efficient containment of substrate H(2)O(2) in the heme cavity arising from easier escape through the more open entrance to the lateral channel created by the smaller side chains of Gly and Ala. Inserting a Cys at position 274 resulted in the heme being covalently linked to the protein through a Cys-vinyl bond that is hypersensitive to X-ray irradiation being largely degraded within seconds of exposure to the X-ray beam. Two heme orientations, flipped along the propionate-vinyl axis, are found in the Ala, Val, and Cys variants.

PubMed: 21332158
DOI: 10.1021/bi200027v

実験手法

X-RAY DIFFRACTION (1.48 Å)