3P91
Crystal structure of Proliferating Cellular Nuclear Antigen from Entamoeba histolytica
Summary for 3P91
| Entry DOI | 10.2210/pdb3p91/pdb |
| Descriptor | Proliferating cell nuclear antigen (2 entities in total) |
| Functional Keywords | dna binding protein, dna replication, processivity, sliding clamp |
| Biological source | Entamoeba histolytica |
| Cellular location | Nucleus : C4M9R9 |
| Total number of polymer chains | 1 |
| Total formula weight | 28822.95 |
| Authors | Lara-Gonzalez, S.,Cardona-Felix, C.S.,Brieba, L.G. (deposition date: 2010-10-15, release date: 2011-06-01, Last modification date: 2023-09-06) |
| Primary citation | Cardona-Felix, C.S.,Lara-Gonzalez, S.,Brieba, L.G. Structure and biochemical characterization of proliferating cellular nuclear antigen from a parasitic protozoon. Acta Crystallogr.,Sect.D, 67:497-505, 2011 Cited by PubMed Abstract: Proliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and biochemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa. PubMed: 21636889DOI: 10.1107/S0907444911010547 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
Download full validation report
