3P91

Crystal structure of Proliferating Cellular Nuclear Antigen from Entamoeba histolytica

3P91 の概要

• エントリーDOI: 10.2210/pdb3p91/pdb
• 分子名称: Proliferating cell nuclear antigen (2 entities in total)
• 機能のキーワード: dna binding protein, dna replication, processivity, sliding clamp
• 由来する生物種: Entamoeba histolytica
• 細胞内の位置: Nucleus : C4M9R9
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28822.95

構造登録者

Lara-Gonzalez, S.,Cardona-Felix, C.S.,Brieba, L.G. (登録日: 2010-10-15, 公開日: 2011-06-01, 最終更新日: 2023-09-06)

主引用文献

Cardona-Felix, C.S.,Lara-Gonzalez, S.,Brieba, L.G.
Structure and biochemical characterization of proliferating cellular nuclear antigen from a parasitic protozoon.
Acta Crystallogr.,Sect.D, 67:497-505, 2011

PubMed Abstract: Proliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and biochemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa.

PubMed: 21636889
DOI: 10.1107/S0907444911010547

実験手法

X-RAY DIFFRACTION (2.4 Å)