3P68

Time-dependent and Protein-directed In Situ Growth of Gold Nanoparticles in a Single Crystal of Lysozyme

3P68 の概要

• エントリーDOI: 10.2210/pdb3p68/pdb
• 関連するPDBエントリー: 3P4Z 3P64 3P65 3P66
• 分子名称: Lysozyme C, GOLD 3+ ION (3 entities in total)
• 機能のキーワード: hydrolase, binding sites, bio-nano hybrids, gold nanoparticles, porous materials
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15709.93

構造登録者

Wei, H.,Wang, Z.,Zhang, J.,House, S.,Gao, Y.-G.,Yang, L.,Robinson, H.,Tan, L.H.,Xing, H.,Hou, C.,Robertson, I.M.,Zuo, J.-M.,Lu, Y. (登録日: 2010-10-11, 公開日: 2011-02-09, 最終更新日: 2024-11-20)

主引用文献

Wei, H.,Wang, Z.,Zhang, J.,House, S.,Gao, Y.G.,Yang, L.,Robinson, H.,Tan, L.H.,Xing, H.,Hou, C.,Robertson, I.M.,Zuo, J.M.,Lu, Y.
Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme.
Nat Nanotechnol, 6:93-97, 2011

PubMed Abstract: Gold nanoparticles are useful in biomedical applications due to their distinct optical properties and high chemical stability. Reports of the biogenic formation of gold colloids from gold complexes has also led to an increased level of interest in the biomineralization of gold. However, the mechanism responsible for biomolecule-directed gold nanoparticle formation remains unclear due to the lack of structural information about biological systems and the fast kinetics of biomimetic chemical systems in solution. Here we show that intact single crystals of lysozyme can be used to study the time-dependent, protein-directed growth of gold nanoparticles. The protein crystals slow down the growth of the gold nanoparticles, allowing detailed kinetic studies to be carried out, and permit a three-dimensional structural characterization that would be difficult to achieve in solution. Furthermore, we show that additional chemical species can be used to fine-tune the growth rate of the gold nanoparticles.

PubMed: 21278750
DOI: 10.1038/nnano.2010.280

実験手法

X-RAY DIFFRACTION (1.6 Å)