3P22

Crystal structure of the ENE, a viral RNA stability element, in complex with A9 RNA

3P22 の概要

• エントリーDOI: 10.2210/pdb3p22/pdb
• 分子名称: Core ENE hairpin from KSHV PAN RNA, oligo(A)9 RNA (3 entities in total)
• 機能のキーワード: major groove triple helix, viral non-coding rna, stability element, nucleus, rna
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 63293.34

構造登録者

Mitton-Fry, R.M.,DeGregorio, S.J.,Wang, J.,Steitz, T.A.,Steitz, J.A. (登録日: 2010-10-01, 公開日: 2010-12-08, 最終更新日: 2024-02-21)

主引用文献

Mitton-Fry, R.M.,DeGregorio, S.J.,Wang, J.,Steitz, T.A.,Steitz, J.A.
Poly(A) tail recognition by a viral RNA element through assembly of a triple helix.
Science, 330:1244-1247, 2010

PubMed Abstract: Kaposi's sarcoma-associated herpesvirus produces a highly abundant, nuclear noncoding RNA, polyadenylated nuclear (PAN) RNA, which contains an element that prevents its decay. The 79-nucleotide expression and nuclear retention element (ENE) was proposed to adopt a secondary structure like that of a box H/ACA small nucleolar RNA (snoRNA), with a U-rich internal loop that hybridizes to and protects the PAN RNA poly(A) tail. The crystal structure of a complex between the 40-nucleotide ENE core and oligo(A)(9) RNA at 2.5 angstrom resolution reveals that unlike snoRNAs, the U-rich loop of the ENE engages its target through formation of a major-groove triple helix. A-minor interactions extend the binding interface. Deadenylation assays confirm the functional importance of the triple helix. Thus, the ENE acts as an intramolecular RNA clamp, sequestering the PAN poly(A) tail and preventing the initiation of RNA decay.

PubMed: 21109672
DOI: 10.1126/science.1195858

実験手法

X-RAY DIFFRACTION (2.501 Å)