3P13

Complex Structure of D-ribose Pyranase Sa240 with D-ribose

3P13 の概要

• エントリーDOI: 10.2210/pdb3p13/pdb
• 関連するPDBエントリー: 3P12
• 分子名称: D-ribose pyranase, beta-D-ribopyranose (3 entities in total)
• 機能のキーワード: rbsd, d-ribose pyranase, sa240, carbohydrate metabolism, isomerase
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 66375.89

構造登録者

Wu, M.,Wang, L.,Zang, J. (登録日: 2010-09-29, 公開日: 2011-05-25, 最終更新日: 2023-11-01)

主引用文献

Wang, L.,Wu, M.,Zang, J.
Crystal structure of Sa240: A ribose pyranase homolog with partial active site from Staphylococcus aureus
J.Struct.Biol., 174:413-419, 2011

PubMed Abstract: Ribose is transported into cells in its pyranose form and must be rearranged to its furanose form for further utilization. Ribose pyranase RbsD catalyzes the conversion of ribose from the pyranose to furanose form. This is the key step for substrate supply to ribokinase RbsK, which converts ribose to ribose-5-phosphate for further metabolism. Sequence analysis indicated Sa240 from Staphylococcus aureus was a ribose pyranase homolog. Here we showed that Sa240 formed dimeric structure both in solution and in crystal. S240-ribose complex structure showed a ribose binding site formed by an incomplete active site compared with RbsD. Because the catalytic activity of ribose pyranase depends on its oligomeric state, we propose Sa240 is catalytically inactive in its dimeric structure.

PubMed: 21276853
DOI: 10.1016/j.jsb.2011.01.007

実験手法

X-RAY DIFFRACTION (2.35 Å)