3OY8
Crystal structure of human galectin-1 in complex with lactobionic acid
Summary for 3OY8
| Entry DOI | 10.2210/pdb3oy8/pdb |
| Related | 3OYW |
| Descriptor | Galectin-1, beta-D-galactopyranose-(1-4)-D-gluconic acid, ... (4 entities in total) |
| Functional Keywords | carbohydrate binding protein, galectin, lactobionic acid |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 2 |
| Total formula weight | 30191.90 |
| Authors | Blanchard, H.,Collins, P.M. (deposition date: 2010-09-23, release date: 2010-11-03, Last modification date: 2024-11-20) |
| Primary citation | Stannard, K.A.,Collins, P.M.,Ito, K.,Sullivan, E.M.,Scott, S.A.,Gabutero, E.,Darren Grice, I.,Low, P.,Nilsson, U.J.,Leffler, H.,Blanchard, H.,Ralph, S.J. Galectin inhibitory disaccharides promote tumour immunity in a breast cancer model CANCER LETT., 2010 Cited by PubMed Abstract: High level galectin-1 expression results in cancer cell evasion of the immune response, increased tumour survival and aggressive metastases. Using a galectin-1 polyclonal antibody, high levels of galectin-1 protein were shown to be expressed by breast cancer cells established from FVB/N MMTV-c-neu mice as well as by the B16F10 melanoma cell line. In mixed lymphocyte cultures using tumour cells as antigenic stimulators, addition of recombinant galectin-1 dose-dependently inhibited lymphocyte production. Disaccharides were identified that inhibited galectin-1 function and increased growth and activation of CD8(+) CTL's killing cancer cells. X-ray crystallographic structures of human galectin-1 in complex with inhibitory disaccharides revealed their mode of binding. Combining galectin-blocking carbohydrates as adjuvants with vaccine immunotherapy in vivo to promote immune responses significantly decreased tumour progression and improved the outcomes for tumour challenged mice. This is the first report showing that suitably selected galectin-1 blocking disaccharides will act as adjuvants promoting vaccine stimulated immune responses against tumours in vivo. PubMed: 20826047DOI: 10.1016/j.canlet.2010.08.005 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.19 Å) |
Structure validation
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