3OY2
Crystal structure of a putative glycosyltransferase from Paramecium bursaria Chlorella virus NY2A
Summary for 3OY2
Entry DOI | 10.2210/pdb3oy2/pdb |
Related | 3OY7 |
Descriptor | Glycosyltransferase B736L (2 entities in total) |
Functional Keywords | rossmann fold, glycosyltransferase, gdp-mannose, sugar, virus capsid proteins, viral protein, transferase |
Biological source | Paramecium bursaria Chlorella virus NY2A (PBCV-NY2A) |
Total number of polymer chains | 2 |
Total formula weight | 93358.59 |
Authors | Xiang, Y.,Rossmann, M.G. (deposition date: 2010-09-22, release date: 2010-11-17, Last modification date: 2024-02-21) |
Primary citation | Xiang, Y.,Baxa, U.,Zhang, Y.,Steven, A.C.,Lewis, G.L.,Van Etten, J.L.,Rossmann, M.G. Crystal structure of a virus-encoded putative glycosyltransferase J.Virol., 84:12265-12273, 2010 Cited by PubMed Abstract: The chloroviruses (family Phycodnaviridae), unlike most viruses, encode some, if not most, of the enzymes involved in the glycosylation of their structural proteins. Annotation of the gene product B736L from chlorovirus NY-2A suggests that it is a glycosyltransferase. The structure of the recombinantly expressed B736L protein was determined by X-ray crystallography to 2.3-Å resolution, and the protein was shown to have two nucleotide-binding folds like other glycosyltransferase type B enzymes. This is the second structure of a chlorovirus-encoded glycosyltransferase and the first structure of a chlorovirus type B enzyme to be determined. B736L is a retaining enzyme and belongs to glycosyltransferase family 4. The donor substrate was identified as GDP-mannose by isothermal titration calorimetry and was shown to bind into the cleft between the two domains in the protein. The active form of the enzyme is probably a dimer in which the active centers are separated by about 40 Å. PubMed: 20861263DOI: 10.1128/JVI.01303-10 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.31 Å) |
Structure validation
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