3OY2

Crystal structure of a putative glycosyltransferase from Paramecium bursaria Chlorella virus NY2A

3OY2 の概要

• エントリーDOI: 10.2210/pdb3oy2/pdb
• 関連するPDBエントリー: 3OY7
• 分子名称: Glycosyltransferase B736L (2 entities in total)
• 機能のキーワード: rossmann fold, glycosyltransferase, gdp-mannose, sugar, virus capsid proteins, viral protein, transferase
• 由来する生物種: Paramecium bursaria Chlorella virus NY2A (PBCV-NY2A)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93358.59

構造登録者

Xiang, Y.,Rossmann, M.G. (登録日: 2010-09-22, 公開日: 2010-11-17, 最終更新日: 2024-02-21)

主引用文献

Xiang, Y.,Baxa, U.,Zhang, Y.,Steven, A.C.,Lewis, G.L.,Van Etten, J.L.,Rossmann, M.G.
Crystal structure of a virus-encoded putative glycosyltransferase
J.Virol., 84:12265-12273, 2010

PubMed Abstract: The chloroviruses (family Phycodnaviridae), unlike most viruses, encode some, if not most, of the enzymes involved in the glycosylation of their structural proteins. Annotation of the gene product B736L from chlorovirus NY-2A suggests that it is a glycosyltransferase. The structure of the recombinantly expressed B736L protein was determined by X-ray crystallography to 2.3-Å resolution, and the protein was shown to have two nucleotide-binding folds like other glycosyltransferase type B enzymes. This is the second structure of a chlorovirus-encoded glycosyltransferase and the first structure of a chlorovirus type B enzyme to be determined. B736L is a retaining enzyme and belongs to glycosyltransferase family 4. The donor substrate was identified as GDP-mannose by isothermal titration calorimetry and was shown to bind into the cleft between the two domains in the protein. The active form of the enzyme is probably a dimer in which the active centers are separated by about 40 Å.

PubMed: 20861263
DOI: 10.1128/JVI.01303-10

実験手法

X-RAY DIFFRACTION (2.31 Å)