3OXJ
crystal structure of glycine riboswitch, soaked in Ba2+
Summary for 3OXJ
| Entry DOI | 10.2210/pdb3oxj/pdb |
| Related | 3OWI |
| Descriptor | domain II of glycine riboswitch, GLYCINE, BARIUM ION, ... (5 entities in total) |
| Functional Keywords | gene expression regulator, glycine riboswitch, rna |
| Total number of polymer chains | 2 |
| Total formula weight | 61426.57 |
| Authors | Huang, L.,Serganov, A.,Patel, D.J. (deposition date: 2010-09-21, release date: 2010-12-29, Last modification date: 2024-03-13) |
| Primary citation | Huang, L.,Serganov, A.,Patel, D.J. Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch. Mol.Cell, 40:774-786, 2010 Cited by PubMed Abstract: Glycine riboswitches regulate gene expression by feedback modulation in response to cooperative binding to glycine. Here, we report on crystal structures of the second glycine-sensing domain from the Vibrio cholerae riboswitch in the ligand-bound and unbound states. This domain adopts a three-helical fold that centers on a three-way junction and accommodates glycine within a bulge-containing binding pocket above the junction. Glycine recognition is facilitated by a pair of bound Mg(2+) cations and governed by specific interactions and shape complementarity with the pocket. A conserved adenine extrudes from the binding pocket and intercalates into the junction implying that glycine binding in the context of the complete riboswitch could impact on gene expression by stabilizing the riboswitch junction and regulatory P1 helix. Analysis of riboswitch interactions in the crystal and footprinting experiments indicates that adjacent glycine-sensing modules of the riboswitch could form specific interdomain interactions, thereby potentially contributing to the cooperative response. PubMed: 21145485DOI: 10.1016/j.molcel.2010.11.026 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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